Simple Assay for Accurate Determination of [ 35 S]sulfate Reduction Activity

A simple and accurate spectrophotometric assay for phosphoenolpyruvate carboxylase activity.

The rate of phosphoenolpyruvate carboxylase activity measured through the conventional coupled assay with malate dehydrogenase is underestimated due to the instability of oxaloacetate, which undergoes partial decarboxylation into pyruvate in the presence of metal ions. The addition of lactate dehydrogenase to the conventional assay allows the reduction of pyruvate formed from oxaloacetate to la...

Simple and Accurate Detection of Vibrio Cholera Using Triplex Dot Blotting Assay

Cholera outbreak is more common in developing countries. The causative agent of the disease is Vibrio cholerae strains O1 and O139. Traditional diagnostic testing for Vibrio is not always reliable, because Vibrio can enter a viable but non cultivable state. Therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing.       In this investigation, a...

Accurate Determination of Optimal Amount of Charger Capacitors for PHEVs

Nowadays, because of the need to decrease fuel consumption and greenhouse gas emissions and also their capability of exchanging power with the grid and regulating the frequency and voltage of the grid at different times of the day, electric vehicles are used more than before all across the world. It is highly important to pay attention to the efficiency, and decrease in the weight and volume of...

A simple method for the determination of serum cholesterol

Simple assay for glycerophospholipid hydrolase activity of postheparin plasma.

An assay using radioactive substrates is described that permits rapid determinations of glycerophospholipid-hydrolyzing activity in postheparin plasma or its fractions. Optimal conditions are described for hydrolysis of phosphatidylcholine and phosphatidylethanolamine. A minimum of only 2 mul of normal postheparin plasma is used and no extraction of the reaction products is required before thei...